Coding

Part:BBa_K3784001:Design

Designed by: LUXUAN LIU   Group: iGEM21_BNUZ-China   (2021-09-19)


fldB (Encoding 2-hydroxyacyl-CoA dehydratase protein family)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
    Illegal BglII site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid.

Source

From fld gene cluster of Clostridium sporogenes

References